- Sic1 Mutants
- sic1∆
- sic1∆ cdh1∆
- sic1∆ cdh1∆ GALL-CDC20
- sic1∆ cdc6∆2-49
- sic1∆ cdc6∆2-49 cdh1∆
- sic1∆ cdc6∆2-49 cdh1∆ GALL-CDC20
- GAL-CLB2 sic1∆
- GAL-CLB5 sic1∆
- CLB5-db∆ sic1∆
- APC-A sic1∆
- APC-A sic1∆ cdc6∆2-49
- cdc14-ts sic1∆
- cln1∆ cln2∆ sic1∆
- cln3∆ bck2∆ sic1∆
- cln1∆ cln2∆ cln3∆ sic1∆
- swi5∆
- swi5∆ GAL-CLB2
- swi5∆ cdh1∆
- swi5∆ cdh1∆ GAL-SIC1
- GAL-SIC1
- GAL-SIC1-db∆
- GAL-SIC1 cln1∆ cln2∆
- GAL-SIC1 GAL-CLN2 cln1∆ cln2∆
- GAL-SIC1 cln1∆ cln2∆ cdh1∆
- GAL-SIC1 GAL-CLN2 cln1∆ cln2∆ cdh1∆
- CLB2-db∆ multicopy SIC1
- CLB2-db∆ GAL-SIC1
- cdc14-ts GAL-SIC1
- cdc14ts then GAL-SIC1
- APC-A cdh1∆ GAL-SIC1
- APC-A cdh1∆ multi-copy SIC1
cln1∆ cln2∆ cln3∆ sic1∆
debug: ,
test user =
test db =
Simulation:
Change of parameters: ksn2'= ksn2"=0, Dn3=0, ksc1'=ksc1"=0.
Length of G1 phase: 5.8 min.
Mass at division: 5.8.
Experiments:
Schneider, B.L., Yang, Q.-H. and Futcher, A.B. (1996). Linkage of replication to Start by the Cdk inhibitor Sic1. Science 272:560-562.
[Abstract] [Article]
[Abstract] [Article]
Tyers, M. (1996). The cyclin-dependent kinase inhibitor p40SIC1 imposes the requirement for Cln G1 cyclin function at Start. Proc. Natl. Acad. Sci. USA 93:7772-7776.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Tyers: Fig. 2, viable with short G1 and very large cell size. Schneider: Fig. 3, viable.
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In the absence of Sic1, although Clb5 kinase are able to initiate DNA synthesis early and to inactivate Cdh1, but to free Clb2 kinase from the inhibition by Cdc6, cells have to grow large enough to accumulate enough Clb5 to do the job. Only after Cdc6 is inhibited, can Clb2 kinase activity appear, and that triggers the positive feedback on its own synthesis and drives the cells into mitosis.